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3i ,3D À̹Ì¡ Çö¹Ì°æ ,diSPIM
ÆǸŰ¡°Ý  : °¡°Ý¹®ÀÇ [VATÆ÷ÇÔ]
ºê·£µå  : Intelligent Imaging Innovation
Á¦Á¶»ç  : Usa
Á¦Ç°Äڵ堠: diSPIM
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Dual-View Inverted Selective Plane Illumination (diSPIM)Àº 3D À̹Ì¡¿¡¼­ ³·Àº ±¤ µ¶¼ºÀ» Á¦°øÇÏ´Â °¡º­¿î ½ÃÆ® ¹æ¹ýÀ¸·Î Àå½Ã°£ µ¿¾È »ì¾ÆÀÖ´Â ÀÛÀº À¯±âü¸¦ À̹ÌÁöÈ­ ÇÒ ¼ö ÀÖ½À´Ï´Ù. Ưº°ÇÑ ½½¶óÀÌµå ¶Ç´Â ¸ð¼¼°üÀÌ ÇÊ¿ä¾ø´Â °³¹æ µÈ Ç¥º» Çü»óÀ» °®Ãá À¯¿¬ÇÑ ¶óÀÌÆ® ½ÃÆ® ½Ã½ºÅÛÀÔ´Ï´Ù. diSPIMÀº Á¶¸í ¹× À̹Ì¡ ¸ðµÎ¿¡ ´ëÇØ ¼øÂ÷ÀûÀ¸·Î »ç¿ëµÇ´Â µÎ °³ÀÇ µî°¡ ±¤ÇÐ °æ·Î¿Í ´ë¹° ·»Áî¿¡¼­ µÎ °³ÀÇ ¼öÁ÷ ¶óÀÌÆ® ½ÃÆ®¸¦ »ç¿ëÇÏ¿© Ç¥º»À» À̹ÌÁöÈ­ÇÕ´Ï´Ù. °á°ú·Î ³ª¿Â 3D µ¥ÀÌÅÍ ¼¼Æ®´Â X, Y ¹× Z¿¡¼­ µî¹æ¼º Çػ󵵸¦ °¡Áö¸ç µÎ °³ÀÇ ºä¸¸ÀÖ¾î ºü¸¥ »ùÇà ĸó°¡ °¡´ÉÇÏ°í »ùÇÃÀÇ »ýÁ¸·ÂÀ» ´Ã¸± ¼ö ÀÖ½À´Ï´Ù.

ÀÏÄ¡ÇÏ´Â ¹° ħÀû ´ë¹° µÎ °³´Â È¿À²ÀûÀÎ Á÷°¢ ÀåÂøÀÌ °¡´ÉÇÑ ÀÛµ¿ °Å¸®·Î ÃÖ´ë 0.8ÀÇ NA¸¦ °¡Áú ¼ö ÀÖ½À´Ï´Ù. °¢ ´ë¹° ·»Áî´Â µ¿ÀÏÇÑ °í¼Ó °íÇØ»óµµ sCMOS Ä«¸Þ¶ó¸¦ ÅëÇØ ¿©±â ¹× ¹æÃâ ¼öÁý¿¡ »ç¿ëµË´Ï´Ù. Á¦ 3 ´ë¹° ·»Áî´Â ½Ã·á¸¦ À§Ä¡½ÃÅ°±â À§ÇØ ½Ã·á è¹ö ¾Æ·¡¿¡ À§Ä¡ÇÑ´Ù. ±¤Àü Á¶ÀÛÀº Ablate ±¤ Àý°³ ½Ã½ºÅÛ°ú °°Àº ¼¼ ¹ø° ¸ñÇ¥¸¦ ÅëÇØ Ãß°¡ ÇÒ ¼ö ÀÖ½À´Ï´Ù.

diSPIMÀº Marianas ½Ã½ºÅÛÀÇ ±¸¼º ¿ä¼Ò, ±âÁ¸ÀÇ ¿¬±¸¿ë °Å²Ù·Î µÈ Çö¹Ì°æ¿¡ Ãß°¡µÇ°Å³ª µ¶¸³ ½ÇÇà Çü ½Ã½ºÅÛÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù. °Å²Ù·Î µÈ Çö¹Ì°æ¿¡ Ãß°¡Çϸé Åõ°ú±¤ °æ·Î°¡ diSPIM ¾î¼Àºí¸®·Î ±³Ã¼µÇ°í Çö¹Ì°æÀÇ ´ë¹° ·»Áî°¡ »ùÇà À§Ä¡¸¦ °áÁ¤ÇÏ´Â µ¥ »ç¿ëµË´Ï´Ù.

SlideBook ¢â 6 ¼ÒÇÁÆ®¿þ¾î´Â diSPIM Çö¹Ì°æ, ·¹ÀÌÀú ½ÇÇà ¹× sCMOS Ä«¸Þ¶ó¸¦ Á¦¾îÇÏ¿© ¸ðµç Çϵå¿þ¾î ±¸¼º ¿ä¼ÒÀÇ º¹ÀâÇÑ °í¼Ó µ¿±âÈ­°¡ °¡´ÉÇÏ¸ç ¿ø½Ã µ¥ÀÌÅ͸¦ µðÄÜ º¼ ·çÆÃÇÏ°í »ùÇÃÀÇ 3D ·»´õ¸µÀ» º¼ ¼ö ÀÖ½À´Ï´Ù. 

Dual-view Inverted Selective Plane Illumination (diSPIM) is a light sheet method offering low phototoxicity in 3D imaging making it possible to image living small organisms over extended periods of time. It is a flexible light sheet system with an open specimen geometry not requiring special slides or capillaries. diSPIM images a specimen using two perpendicular light sheets from two equivalent optical paths and objectives which are each used sequentially for both illumination and imaging. The resulting 3D datasets have isotropic resolution in X, Y and Z with only two views, resulting in fast image capture with low light dose for extended specimen viability.

Two matching water dipping objectives can have an NA of up to 0.8 with a working distance allowing efficient orthogonal mounting. Each objective is used for excitation and emission collection via identical high-speed high-resolution sCMOS cameras. A third objective is located below the specimen chamber for locating the sample. Photomanipulation can be added via the third objective, such as the Ablate photoablation system.

diSPIM is available as a component for the Marianas system, an addition to an existing research inverted microscope, or as a stand-alone system. When added to an inverted microscope the transmitted light path is replaced by the diSPIM assembly and the microscope's objective is used for locating the sample.

SlideBook¢â 6 software controls the diSPIM microscope, laser launch and sCMOS cameras allowing for intricate high-speed synchronization of all hardware components along with the ability to deconvolve raw data and view 3D renderings of the sample

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